Determination of the Predatory Capability of Bdellovibrio bacteriovorus HD100

Determination of the Predatory Capability of Bdellovibrio bacteriovorus HD100

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Bdellovibrio bacteriovorus HD100 is an obligate predator that preys upon a wide variety of Gram negative bacteria.The biphasic growth cycle of Bdellovibrio POWDER FOUNDATION VANILLA #1110 includes a free-swimming attack phase and an intraperiplasmic growth phase, where the predator replicates its DNA and grows using the prey as a source of nutrients, finally dividing into individual cells (Sockett, 2009).Due to its obligatory predatory lifestyle, manipulation of Bdellovibrio requires two-member culturing techniques using selected prey microorganisms (Lambert et al., 2003).

In this protocol, we describe a detailed workflow to grow and quantify B.bacteriovorus HD100 and its predatory ability, to easily Card Organizer carry out these laborious and time-consuming techniques.